Rapid ultramicro estimation of serum total cholesterol
نویسندگان
چکیده
منابع مشابه
A rapid and specific ultramicro method for total serum cholesterol.
A modification of the Zak cholesterol reaction is described in which ethanol is substituted for glacial acetic acid. This modification avoids the hazard of purifying glacial acetic acid and also increases the specificity of the reaction by eliminating ir.terference from bromide or iodide salts. The ethanolic reaction has the absorbance spectrum, sensitivity, and stability of the usual acetic ac...
متن کاملAn ultramicro method for the estimation of plasma cholesterol.
A procedure is outlined for the ultramicro determination of total cholesterol that gives results in excellent agreement with classic methods. The determination requires 40 l. of plasma obtained from a finger-tip puncture and special handling of precipitated proteins. By the use of the described procedure plasma cholesterol levels have been estimated 4 times daily over a period of 8 weeks in you...
متن کاملEstimation of total cholesterol in serum by a micro method.
LEERS AND Lowa (1) HAVE PUBLISHED a method for the estimation of cholesterol on a micro scale in animal tissue by fluorometry involving the reaction of extracted cholesterol dissolved in trichloroethane with acetic anhydride and sulfuric acid. The present paper describes a simple and tested use of this reaction for the estimation of total cholesterol in 0.02 ml. of serum after extraction by a s...
متن کاملThe remonal effect of hydrogenated shortening on serum levels of triglycerids,total cholesterol and HDL-cholesterol in normal subjects
In Iran,the hydrogenated vegetable oil have content of frans fatty acids and high per capita consumption.this study was aimed to evaliate the effects of substitution of hydrogenated oil by sunflower liquid oil on plasma levels of LDL,HDL, total cholestero and triglycerids in normal subjects.thirty six healthy inmates participated in this research. they were consuming hydrogenated oil before the...
متن کاملEnzymatic determination of total serum cholesterol.
An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the Calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1960
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39059-3